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GEXSCOPE® Single Nucleus RNA Library Kit

Single cell RNA sequencing usually requires as an input a single cell suspension prepared from fresh samples. For single cell sequencing of frozen tissues, we offer a specialized product, the GEXSCOPE® Single Nucleus RNA Library Kit. The GEXSCOPE® Single Nucleus RNA Library Kit is a complete solution for transforming tissues into single nuclei and converting the mRNA contained in the single nuclei into NGS libraries.  

The GEXSCOPE® Single Nucleus RNA Library Kit can not only be used to analyze frozen tissue samples, but it can also be applied for single nucleus sequencing of the frozen cells isolated by FACS sorting. In addition, the GEXSCOPE® Single Nucleus RNA Library Kit can also be utilized for the single cell analysis of the cells with a large diameter or irregular shape such as neurons.  

Workflow
 
 
Advantages of the GEXSCOPE® Single Nucleus RNA Library Kit
Example of an Experimental Dataset

Frozen hippocampus tissue from mouse brain was processed using the GEXSCOPE® Single Nucleus RNA Library Kit. After nucleus isolation, partitioning and barcoding, a single nucleus library was sequenced, and the obtained data were clustered. Based on the expression profiles of marker genes, major cell subtypes (excitatory neurons, inhibitory neurons, glial cells, neuroblasts and oligodendrocytes) were annotated using the SynEcoSys database.

 
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Phone:+49 221 16824777

E-mail:info@singleronbio.com (German office)

Address:Singleron Biotechnologies GmbH, Gottfried-Hagen-Strasse 60, 51105 Cologne, Germany

VAT number:DE334592111

District Court Cologne, HRB 104037

Managing director:Dr. Nan Fang | Dr. Bruno Monschau