GEXSCOPE® Single Cell RNA Library Kit GEXSCOPE® Single Nucleus RNA Library Kit NEW! - GEXSCOPE® Microbial Single Cell RNA Library Kit HD (Yeast) GEXSCOPE® Single Cell V(D)J Kit DynaSCOPE™ Single Cell Dynamics RNA Library Kit FocuSCOPE™ Single Cell Targeted Capture Kit sCircle™ Single Cell Full-length TCR sequencing CLindex™ Sample Multiplexing Kit NEW! - sCelLiVE™ Tissue to Living Single Cell Suspension Kit NEW! - AccuraCode® RNA Library Construction Kit
Singleron's sCircle technology enables full-length V(D)J reagion sequencing at single cell level with short-read sequencing.
Using Singleron's GEXSCOPE® technology, single cells are partitioned into individual microwells, and mRNAs are captured by uniquely barcoded beads. The mRNA are then reverse transcribed into cDNA, followed by a circularization step introduced by a circle handle. By circularization of cDNA, the V(D)J region was brought closer to the sequencing primer. After two rounds of enrichments, the products will be fragmented and constructed for full-length VDJ library for illumina short-read sequencing.
VDJ recombination is the process by which T cell and B cells randomely assemble different gene segments – known as variable (V), diversity (D) and joining (J) genes – in order to generate unique receptors (known as antigen receptors) that can collectively recognize many different types of molecule. The V(D)J region is one of the most highly diversed region on human genome, contributing to complex human immune responses. Therefore understanding the full-length sequences of T cell V(D)J regions provided a more precise understanding towards the immune processes.
Tumor Infiltrating T cells
Identify abnormal T cells in Immune System
Identify rejection risks for transplantation
Development of Immunetherapies