DynaSCOPE™ single cell transcription dynamics detection kit is the one-and-only commercial kits available that measures the rate and level of nascent RNA for an individual gene at a given time point in a single cell.
By labeling the RNA synthesis intermediates and parallel analysis of thousands of cells for their gene expression level, DynaSCOPE™ can differentiate the “new” transcript from the “old” transcript, and enables the measurement of the kinetics of RNA transcription and degradation in every single cell.
Transcription dynamics has opened up new ways of studying cellular gene expression events and regulations, by adding a third dimension-- the “time” scale-- to our current understandings.
What can DynaSCOPE™ analyze:
1. Detecting nascent RNA level and RNA synthesis rate.
2. Monitoring the degradation rate of RNA.
3. Analyzing the transcription dynamics and regulation.
4. Research on transcription regulation.
First High-Throughput Single-Cell Transcriptome Dynamics Research Tool
Fig. Mouse lung tissue were analyzed using DynaSCOPE™ transcription dynamics kit after 2hrs labeling.
(A) shows RNA transcriptional activity (the darker, the more active) in different cell clusters.
(B) The percentage of nascent RNA (newly transcribed RNA) in different genes (upper panel) and in different cell types (lower panel).